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selection marker  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology selection marker
    Selection Marker, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/selection+marker/CASC3+CRISPR%2FCas9+KO+Plasmid/pmc12997282-156-15-28
    Average 94 stars, based on 1 article reviews
    selection marker - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Selection:

    Article Title: Neuroprotective effects of osmotin in Parkinson’s disease-associated pathology via the AdipoR1/MAPK/AMPK/mTOR signaling pathways
    Article Snippet: Adiponectin receptor (AdipoR1) was knocked out by a commercial AdipoR1 CRISPR/Cas9-KO plasmid (Santa Cruz, CA, USA). .. The insertion of the puromycin gene as a selection marker was performed using an AdipoR1 HDR plasmid (Santa Cruz, CA, USA). .. Cells were transfected using the abovementioned plasmids and Lipofectamine 3000 (Invitrogen, CA, USA) according to the instructions provided by the manufacturer [ ].

    Article Title: MLN51 (Metastatic Lymph Node Gene 51)/CASC3 (Cancer Susceptibility Candidate Gene 3), A Putative Tumour Suppressor in Breast Cancer, the Clinical and Therapeutic Connections
    Article Snippet: After transfection and validation, cells were subjected to selection with 1 μg/mL puromycin (Fisher Scientific, Oxford, UK) and, once tested for the success of genetic modification, were routinely maintained in maintenance medium (with 0.1 μg/mL puromycin). .. A human MLN51 CRISPR transcription activation system, which comprised three plasmids, each carrying a different selection marker, allowing the transcriptional activation of MLN51 (sc-411606), was also purchased from Santa Cruz Biotechnology (Santa Cruz, TX, USA). .. MDA MB-231 and MDA MB-361 breast cancer cells were transfected with the human MLN51 CRISPR activation plasmids in T25 flasks for 24 h. After transfection and validation, the cells were subjected to selection using triple antibiotics, including 2 μg/mL puromycin (Fisher Scientific, Oxford, UK), 200 μg/mL Hygromycin B (Fisher Scientific, Oxford, UK), and 1 μg/mL Blasticidin S HCl (Fisher Scientific, Oxford, UK).

    Marker:

    Article Title: Neuroprotective effects of osmotin in Parkinson’s disease-associated pathology via the AdipoR1/MAPK/AMPK/mTOR signaling pathways
    Article Snippet: Adiponectin receptor (AdipoR1) was knocked out by a commercial AdipoR1 CRISPR/Cas9-KO plasmid (Santa Cruz, CA, USA). .. The insertion of the puromycin gene as a selection marker was performed using an AdipoR1 HDR plasmid (Santa Cruz, CA, USA). .. Cells were transfected using the abovementioned plasmids and Lipofectamine 3000 (Invitrogen, CA, USA) according to the instructions provided by the manufacturer [ ].

    Article Title: MLN51 (Metastatic Lymph Node Gene 51)/CASC3 (Cancer Susceptibility Candidate Gene 3), A Putative Tumour Suppressor in Breast Cancer, the Clinical and Therapeutic Connections
    Article Snippet: After transfection and validation, cells were subjected to selection with 1 μg/mL puromycin (Fisher Scientific, Oxford, UK) and, once tested for the success of genetic modification, were routinely maintained in maintenance medium (with 0.1 μg/mL puromycin). .. A human MLN51 CRISPR transcription activation system, which comprised three plasmids, each carrying a different selection marker, allowing the transcriptional activation of MLN51 (sc-411606), was also purchased from Santa Cruz Biotechnology (Santa Cruz, TX, USA). .. MDA MB-231 and MDA MB-361 breast cancer cells were transfected with the human MLN51 CRISPR activation plasmids in T25 flasks for 24 h. After transfection and validation, the cells were subjected to selection using triple antibiotics, including 2 μg/mL puromycin (Fisher Scientific, Oxford, UK), 200 μg/mL Hygromycin B (Fisher Scientific, Oxford, UK), and 1 μg/mL Blasticidin S HCl (Fisher Scientific, Oxford, UK).

    Plasmid Preparation:

    Article Title: Neuroprotective effects of osmotin in Parkinson’s disease-associated pathology via the AdipoR1/MAPK/AMPK/mTOR signaling pathways
    Article Snippet: Adiponectin receptor (AdipoR1) was knocked out by a commercial AdipoR1 CRISPR/Cas9-KO plasmid (Santa Cruz, CA, USA). .. The insertion of the puromycin gene as a selection marker was performed using an AdipoR1 HDR plasmid (Santa Cruz, CA, USA). .. Cells were transfected using the abovementioned plasmids and Lipofectamine 3000 (Invitrogen, CA, USA) according to the instructions provided by the manufacturer [ ].

    CRISPR:

    Article Title: MLN51 (Metastatic Lymph Node Gene 51)/CASC3 (Cancer Susceptibility Candidate Gene 3), A Putative Tumour Suppressor in Breast Cancer, the Clinical and Therapeutic Connections
    Article Snippet: After transfection and validation, cells were subjected to selection with 1 μg/mL puromycin (Fisher Scientific, Oxford, UK) and, once tested for the success of genetic modification, were routinely maintained in maintenance medium (with 0.1 μg/mL puromycin). .. A human MLN51 CRISPR transcription activation system, which comprised three plasmids, each carrying a different selection marker, allowing the transcriptional activation of MLN51 (sc-411606), was also purchased from Santa Cruz Biotechnology (Santa Cruz, TX, USA). .. MDA MB-231 and MDA MB-361 breast cancer cells were transfected with the human MLN51 CRISPR activation plasmids in T25 flasks for 24 h. After transfection and validation, the cells were subjected to selection using triple antibiotics, including 2 μg/mL puromycin (Fisher Scientific, Oxford, UK), 200 μg/mL Hygromycin B (Fisher Scientific, Oxford, UK), and 1 μg/mL Blasticidin S HCl (Fisher Scientific, Oxford, UK).

    Activation Assay:

    Article Title: MLN51 (Metastatic Lymph Node Gene 51)/CASC3 (Cancer Susceptibility Candidate Gene 3), A Putative Tumour Suppressor in Breast Cancer, the Clinical and Therapeutic Connections
    Article Snippet: After transfection and validation, cells were subjected to selection with 1 μg/mL puromycin (Fisher Scientific, Oxford, UK) and, once tested for the success of genetic modification, were routinely maintained in maintenance medium (with 0.1 μg/mL puromycin). .. A human MLN51 CRISPR transcription activation system, which comprised three plasmids, each carrying a different selection marker, allowing the transcriptional activation of MLN51 (sc-411606), was also purchased from Santa Cruz Biotechnology (Santa Cruz, TX, USA). .. MDA MB-231 and MDA MB-361 breast cancer cells were transfected with the human MLN51 CRISPR activation plasmids in T25 flasks for 24 h. After transfection and validation, the cells were subjected to selection using triple antibiotics, including 2 μg/mL puromycin (Fisher Scientific, Oxford, UK), 200 μg/mL Hygromycin B (Fisher Scientific, Oxford, UK), and 1 μg/mL Blasticidin S HCl (Fisher Scientific, Oxford, UK).



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